{"id":36161,"date":"2025-01-21T15:04:47","date_gmt":"2025-01-21T20:04:47","guid":{"rendered":"https:\/\/www.eng.ufl.edu\/newengineer\/?p=36161"},"modified":"2025-12-16T15:03:19","modified_gmt":"2025-12-16T20:03:19","slug":"uf-engineers-develop-new-protocol-to-purify-dna","status":"publish","type":"post","link":"https:\/\/www.eng.ufl.edu\/news\/stories\/uf-engineers-develop-new-protocol-to-purify-dna\/","title":{"rendered":"UF engineers develop new protocol to purify DNA"},"content":{"rendered":"\n

When you think about scientific research, you probably envision a sterile room that houses gleaming beakers, rows of pipettes and centrifuges. But what if you could house a lab on<\/em> a chip?<\/p>\n\n\n\n

Call it the science of small, but a trio of University of Florida chemical engineers have developed a lab-on-a-chip process that could make a big difference for DNA research and, ultimately, patients.<\/p>\n\n\n\n

Microfluidic technology miniaturizes complex laboratory processes \u2013 such as mixing, reactions, separations, etc. \u2013 into devices the size of a microscope slide. The UF team has developed a microfluidic device for DNA purification that could speed up analysis of laboratory samples and match or exceed the purity of existing methods.<\/p>\n\n\n

\n
\"\"
This microscope slide-sized device can extract long DNA from a cell sample. (Photo by Dave Schlenker)\n<\/figcaption><\/figure>\n<\/div>\n\n\n

In their paper \u201cMicrofluidic Purification of Genomic DNA,<\/a>\u201d UF professors Jason Butler, Ph.D., and Tony Ladd, Ph.D., along with their fourth-year Ph.D. candidate and research assistant, Jiayi Wang, detailed their new\u00a0 device, which extracts genomic DNA without centrifuges, magnetic beads or gels. The paper was published this week in the Proceedings of the National Academy of Sciences.<\/p>\n\n\n\n

Purification removes contaminants from DNA samples, ensuring the integrity of the genetic material and providing more accurate results.<\/p>\n\n\n\n

To purify DNA, individual cells must be broken open, proteins and other contaminants removed, and the purified DNA recovered. Current extraction protocols often break the DNA molecules into small fragments, due to stresses from centrifuging or from the tension of a flowing fluid pulling on tethered DNA molecules. Butler and Ladd think their microfluidic protocol is gentler and will reduce DNA fragmentation.<\/p>\n\n\n\n

The groundwork for this new device began in 2006 with contributions from several different Ph.D. students, but \u201cit was Jiayi who succeeded in the critical steps of extracting the purified DNA and demonstrating its purity by standard biochemical assays. She deserves a lot of credit for her persistence in overcoming many obstacles,\u201d said Ladd.<\/p>\n\n\n\n

Their device \u2013 the size of a microscope slide \u2013 uses fluid flow and electric fields to push contaminated DNA solutions through a channel about 100 microns square, or roughly the size of a human hair. The fluid flow distorts coiled DNA into elongated shapes, enabling the electric field to push them toward the channel walls.<\/p>\n\n\n\n

\u201cThe DNA moves to the walls of the channel and builds up, while all other components in the sample are carried away by the fluid flow,\u201d said Butler.<\/p>\n\n\n\n

How does this research fit into the bigger picture?<\/p>\n\n\n\n

\u201cDNA sequencing can be used to identify diseases, such as cancer,\u201d Butler explained. \u201cLong-read sequencers can recognize regions of the genome that are hard to identify from short sequences but are suspected to be associated with cancer.\u201d<\/p>\n\n\n\n

Given the ease of sample preparation, microfluidic extraction will likely lead to wider adoption of long-read sequencers, which sequence DNA in long strands without breaking them into smaller fragments.<\/p>\n\n\n\n

Wang will graduate this May, but her work will continue.<\/p>\n\n\n\n

\u201cThe most valuable lesson from this experience was learning how to tackle challenges while maintaining a positive attitude, even in tough situations,\u201d she said. \u201cThese experiences gave me the confidence to pursue a research position after graduation.\u201d<\/p>\n","protected":false},"excerpt":{"rendered":"

A UF team has developed a microfluidic device for DNA purification that could speed up analysis of laboratory samples and match or exceed the purity of existing methods.<\/p>\n","protected":false},"author":2592,"featured_media":36167,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"single-templates\/single-sidebar-none.php","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"featured_post":"off","footnotes":"","_links_to":"","_links_to_target":""},"categories":[11,35,61,57],"tags":[509],"class_list":["post-36161","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-che","category-featured","category-research-innovation","category-stories","tag-topstories2025"],"acf":[],"yoast_head":"\nUF engineers develop new protocol to purify DNA - News from Herbert Wertheim College of Engineering<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.eng.ufl.edu\/news\/stories\/uf-engineers-develop-new-protocol-to-purify-dna\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"UF engineers develop new protocol to purify DNA - 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