{"id":2883,"date":"2017-09-21T12:56:14","date_gmt":"2017-09-21T18:56:14","guid":{"rendered":"https:\/\/www.eng.ufl.edu\/nimet\/?page_id=2883"},"modified":"2026-03-12T10:28:56","modified_gmt":"2026-03-12T14:28:56","slug":"ryosuke-yokosawa-2","status":"publish","type":"page","link":"https:\/\/www.eng.ufl.edu\/nimet\/events\/nanoflorida-conference\/nanoflorida-2017\/ryosuke-yokosawa-2\/","title":{"rendered":"Ryosuke Yokosawa"},"content":{"rendered":"<p><b><a href=\"https:\/\/www.eng.ufl.edu\/nimet\/wp-content\/uploads\/sites\/227\/2017\/09\/Ryosuke-Yokosawa.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2887 alignleft\" src=\"https:\/\/www.eng.ufl.edu\/nimet\/wp-content\/uploads\/sites\/227\/2017\/09\/Ryosuke-Yokosawa.jpg\" width=\"201\" height=\"201\" \/><\/a>2017 NanoFlorida Participant<\/b><br \/>\n<strong>Ph.D. Student\u00a0in the Department of Mechanical Engineering<\/strong><em><br \/>\nM.S., Florida Institute of Technology<br \/>\nB.S., Kogakuin University<br \/>\n<\/em><\/p>\n<p><strong>Biography:<\/strong><\/p>\n<ul>\n<li>Ryosuke graduated with his\u00a0B.S. in Global\u00a0Engineering\/Mechanical Engineering from\u00a0Kogakuin University in 2014 and his\u00a0M.S. in Mechanical Engineering from the\u00a0Florida Institute\u00a0of Technology in 2016. He is currently a PhD student in Mechanical Engineering under the advisement of\u00a0Dr.\u00a0Simmons.\u00a0He\u00a0has\u00a0been\u00a0collaborating\u00a0on mechanobiology research with\u00a0Dr.\u00a0Glover&#8217;s lab in Gastrointestinal Medicine.\u00a0His projects\u00a0include\u00a0image analysis for traction force microscopy (TFM),\u00a0fabrication of polydimethylsiloxane (PDMS) silicone\u00a0for tissue stiffness-matched substrate, and biological analysis for cells on stiffness-matched surface such as qPCR, immuno staining and imaging.<\/li>\n<li>His research interests are\u00a0cellular\u00a0biology,\u00a0biomedical materials,\u00a0and\u00a0image analysis.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><b><a href=\"https:\/\/www.eng.ufl.edu\/nimet\/wp-content\/uploads\/sites\/227\/2017\/09\/Abstract-Image.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-2889 alignright\" src=\"https:\/\/www.eng.ufl.edu\/nimet\/wp-content\/uploads\/sites\/227\/2017\/09\/Abstract-Image-266x300.jpg\" alt=\"\" width=\"353\" height=\"398\" srcset=\"https:\/\/www.eng.ufl.edu\/nimet\/wp-content\/uploads\/sites\/227\/2017\/09\/Abstract-Image-266x300.jpg 266w, https:\/\/www.eng.ufl.edu\/nimet\/wp-content\/uploads\/sites\/227\/2017\/09\/Abstract-Image.jpg 593w\" sizes=\"auto, (max-width: 353px) 100vw, 353px\" \/><\/a>Title:\u00a0<\/b>Tissue stiffness-matched substrates for mechanobiology of intestinal epithelial cells<\/p>\n<p><strong>Authors:<\/strong>\u00a0Ryosuke Yokosawa, MS, Dalton Berrie, MS, Daniel Stewart, MS, Sarah Glover, DO, Chelsey S. Simmons, PhD<\/p>\n<p><span style=\"color: black\"><b>Abstract:\u00a0<\/b>Intestinal epithelial Caco-2 cells are often used in pharmaceutical research to test absorption of various compounds, but rigid plastic transwell surfaces do not match the mechanical properties of intestine. We have used a custom characterization tool to quantify the mechanical properties of human, porcine, bovine, and murine intestine, and we have fabricated soft cell culture surfaces from polydimethylsiloxane (PDMS) to mimic properties of the intestines. Properties of large and small intestines of all patients and animals quantified were 1-10 kPa, and Sylgard 527 was used to match these values (~5 kPa). Sylgard 182 was also used to make an intermediate substrate (~2MPa) softer than plastic (GPa) that is commonly used in other mechanobiology experiments, allowing us to compare results in the literature. We observed that intestine-like stiffness slows cell adhesion compared to the plastic (GPa) control surface, and metabolic (MTT) assay quantified lower activity on PDMS surfaces compared to control plastic. Extracellular matrix related proteins (Col I and Col IV) and enzymes (MMP1 and MMP3) were measured by qPCR, and collagens were downregulated on soft surface and MMP1 was slightly upregulated.<\/span><\/p>\n<p>We are currently comparing these qPCR data to results from patient tissue to determine the relevance of soft PDMS substrates as a biomimetic culture system. Future work will fabricate pores in PDMS so that Caco-2 cells can adequately polarize and more closely reflect epithelial monolayers and ideally crypts of the intestines.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>2017 NanoFlorida Participant Ph.D. Student\u00a0in the Department of Mechanical Engineering M.S., Florida Institute of Technology B.S., Kogakuin University Biography: Ryosuke graduated with his\u00a0B.S. in Global\u00a0Engineering\/Mechanical Engineering from\u00a0Kogakuin University in 2014 and his\u00a0M.S. in Mechanical Engineering from the\u00a0Florida Institute\u00a0of Technology in 2016. He is currently a PhD student in Mechanical Engineering under the advisement of\u00a0Dr.\u00a0Simmons.\u00a0He\u00a0has\u00a0been\u00a0collaborating\u00a0on mechanobiology [&hellip;]<\/p>\n","protected":false},"author":0,"featured_media":0,"parent":3597,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-section-nav.php","meta":{"_acf_changed":false,"inline_featured_image":false,"featured_post":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2883","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.eng.ufl.edu\/nimet\/wp-json\/wp\/v2\/pages\/2883","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.eng.ufl.edu\/nimet\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.eng.ufl.edu\/nimet\/wp-json\/wp\/v2\/types\/page"}],"replies":[{"embeddable":true,"href":"https:\/\/www.eng.ufl.edu\/nimet\/wp-json\/wp\/v2\/comments?post=2883"}],"version-history":[{"count":1,"href":"https:\/\/www.eng.ufl.edu\/nimet\/wp-json\/wp\/v2\/pages\/2883\/revisions"}],"predecessor-version":[{"id":12983,"href":"https:\/\/www.eng.ufl.edu\/nimet\/wp-json\/wp\/v2\/pages\/2883\/revisions\/12983"}],"up":[{"embeddable":true,"href":"https:\/\/www.eng.ufl.edu\/nimet\/wp-json\/wp\/v2\/pages\/3597"}],"wp:attachment":[{"href":"https:\/\/www.eng.ufl.edu\/nimet\/wp-json\/wp\/v2\/media?parent=2883"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}