Novel Approaches for Isolating Hematopoietic Stem Cells Using a Planer Gradient Magnetic System Heather Blackwell and Samuel Hart Authors: Heather A Blackwell, Samuel M Hart, Jon P Dobson Faculty Mentor: Jon Dobson, PhD College: College of Engineering Department: Biomedical Engineering |
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AbstractHematopoietic stem cell transplants are a life-saving treatment performed on patients who suffer from hematological malignancies including leukemias, lymphomas, myelomas, and sickle cell disease. Hematopoietic stem cells (CD34+), a rare population, are isolated from donors (allogenic) or patients (autologous) via bone marrow harvest (less common) or mobilized peripheral blood collection. It has been shown that an increase in CD34+ cells collected for transplantation can decrease the time to engraftment, reduce the potential for transplant related mortality, and increase the overall survival rate [1]. There is a need to develop additional strategies for increasing the CD34+ cell dosage obtained and we have proposed a solution using extracorporeal magnetic filtration of the targeted cell population. Commercially available isolation kits are limited in the volume they can process and require extensive, time-consuming preprocessing steps that we plan to eliminate. The planer flow separation system we have designed has a high throughput capability and increased capture efficiency compared to available commercial models. Our process utilizes magnetic nanoparticles coated with a capture antibody that will be used to bind to the target population then isolated via our separation process. Initial testing will be performed on CD34+ cells in an aqueous solution then progressing to donated human cord blood samples. By validating our magnetic filtration system we will demonstrate the system’s ability to capture target cells bound to the magnetic nanoparticles.
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Poster
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