 Magnetic Separation System for the Isolation of IL-6 and COVID-19 Antibodies Samuel Hart and David Hall Authors: Samuel Hart, David Hall, Stephanie Herrera, Jon Dobson Faculty Mentor: Jon Dobson, PhD College: College of Engineering Department: Biomedical Engineering |
|
AbstractMagnetic separation chambers were created to separate a desired cell type or biomolecule from biological fluids. In these systems, magnetic particles are functionalized with a specific biomolecule that allows them to capture a specific cell type or label a targeted biomolecule. When a solution containing these cells and particles is placed in a high gradient magnetic field, the particles (and the cells or biomolecules to which they are attached) will be attracted to the field. As the solution flows, the particles will remain in the separation chamber while non-labeled cells and the supernatant flow through. Though many magnetic cell separation systems have been developed over the past three decades, none of them offer high throughout, optimization for labeling and separation of cells. Additionally, these systems typically have low capture efficiency. Interleukin 6 (IL-6) acts as a pro-inflammatory cytokine that is commonly associated with modulating the immune response. The objective of this work is to create an optimized magnetic cell separation system that has a larger operational volume and higher capture efficiency which will then be used to capture various biomolecules like IL-6 and COVID-19 antibodies from blood plasma.
|
|
Poster
|
|
